Psv To Mcr Download Figure Open

Dinh Find this author on Google Scholar Find this author on PubMed Search for this author on this site Jasti S.

Rao Find this author on Google Scholar Find this author on PubMed Search for this author on this site.

A, DAOY and D283 cells were transfected with mock (1 PBS), pSV, or siRNA against MMP-9 andor uPAR (pM, pU, and pUM).

After 36 hours of incubation, cells were irradiated with 8 Gy and incubated for a further 12 hours.

Total RNA isolated from the transfected cells were used in determining the expression levels of MMP-9 and uPAR by qRT-PCR method.

Relative expression levels of the gene were graphically represented.

Expression of GAPDH was used to verify uniform levels of cDNA.

B, total cell lysates were used to determine MMP-9 and uPAR levels using Western blot analysis.

C, the band intensities of MMP-9 and uPAR were quantified by densitometry.

Psv To Mcr Download Figure Open

Columns, mean of triplicate experiments; bars, SD; and, P data-icon-position data-hide-link-title0 Download figure Open in new tab Download powerpoint Figure 1.

Columns, mean of triplicate experiments; bars, SD; and, P P 3 DAOY and D283 cells were transfected with mock (1 PBS), pSV, pM, pU, or pUM alone or in combination with IR.

After 36 hours of incubation, cells were irradiated with 8 Gy and incubated for different time intervals (24, 36, 48, 60, and 72 hours).

The percent viability was calculated as a ratio of mean absorbance of sample to mean absorbance of mock multiplied by 100, and values were plotted.

To determine cell cycle arrest, the DNA content of medulloblastoma cells was measured by FACS analysis.

Representative images of (A) DAOY) and (B) D283 from each treatment were given.